Ecosystem Transformation (), 125-135

Bioconversion of plant waste as a tool for regulating the techno-ecosystem of the pulp and paper industry in the North-Western region

Bolotnikova O.I., Kvasha N.V., Malevskaia-Malevich E.D. 

The prospects of introducing a circular economic model for optimizing the techno-ecosystems of the pulp and paper industry (PPI) in the North-West of the Russian Federation are investigated. The potential of biorefining PPI waste (sulfite liquor and lignocellulose hydrolysates) into bioethanol is considered. A comparative analysis of the effectiveness of alcoholic fermentation of D-xylose, the main pentatomic sugar of PPI waste, and various types of xylose-assimilating yeast from domestic collections of industrial microorganisms is presented. The best rate and efficiency of alcohol formation were noted for C. shehatae Y-1632 (0.83 g/l×h and 0.40 g/g of consumed D-xylose), P. stipitis Y-1483
(0.76 g/l×h and 0.39 g/g), and P. tannophilus Y-1533 (0.65 g/l×h and 0.27 g/g). The features of xylose reductase (XR) and xylitol dehydrogenase (XD), key enzymes of D-xylose catabolism, which affect the level of bioethanol production, have been studied. The XR of alcohol-forming yeast had a double NADPH/NADH had coenzyme specificity, whereas XD was characterized by high affinity for NAD+. The highest XR activity was detected for P. stipitis Y-2160 (15.21 mmol/mg×min). C. shehatae Y-1632 (13.95 mmol/mg×min) had the highest XD activity. The maximum permissible values for alcohol-forming activity have been established. C. shehatae Y-1632 and P. tannophilus Y-1533 alcohol concentrations in the
medium: 45.5 g/l and 46 g/l, respectively. P. tannophilus Y-1533 has been shown to be highly resistant to toxic impurities from PPI waste: furfural (F), oxymethylfurfural (OF), volatile organic acids (VOA), and substances of the lignofuran complex (SLC). The maximum permissible concentrations of inhibitors for the growth of these yeasts were (g/l): 0.1 (F); 0.27 (OF), 0.50 (VOA) and 1.74 (SLC). For the first time, the biotechnological potential of Pachysolen tannophilus Y-1533 yeast for optimizing the technoecosystems of the PPI region of presence is discussed.

O. I. Bolotnikova
North-West Institute of Management – branch of The Russian Presidential Academy of National Economy and Public Administration
Sredniy Prospekt V.O. 57/43, St. Petersburg, 199178 Russia

Petrozavodsk State University
Prospekt Lenina St. 33, Petrozavodsk, Republic of Karelia, 185910 Russia


N. V. Kvasha
North-West Institute of Management – branch of The Russian Presidential Academy of National Economy and Public Administration
Sredniy Prospekt V.O. 57/43, St. Petersburg, 199178 Russia

Bonch-Bruevich Saint-Petersburg State University of Telecommunications
Prospekt Bo’shevikov St. 22, build. 1, St. Petersburg, 193232 Russia


E. D. Malevskaia-Malevich
North-West Institute of Management – branch of The Russian Presidential Academy of National Economy and Public Administration
Sredniy Prospekt V.O. 57/43, St. Petersburg, 199178 Russia

malevskaya-ed@ranepa.ru

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